Modification of yeast pyruvate kinase by an active site-directed reagent, bromopyruvate.
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Modification of yeast pyruvate kinase by an active site-directed reagent, bromopyruvate.
The reaction of bromopyruvate with pyruvate kinase was demonstrated both by noting loss of catalytic activity with time and by incorporation of [14C]pyruvate. The first order rate constant for loss of catalytic activity when plotted as a function of bromopyruvate concentration fit a regular hyperbolic saturation function indicative of the formation of a complex with Ki = 15 mM prior to the inac...
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Eby, D. & Kirtley, M. E. (1971) Biochemistry 10,2677-2682 Fuller-Noel, J. K. & Schumaker, V. W. (1972) J. Mol. Biol. 68,523-532 Gennis, L. S. (1976) Proc. Natl. Acad. Sci. U.S.A. 73, 3928-3932 Harrigan, P. J. & Trenthami, D. R. (1971) Biochem. J . 124, 573-580 Hams, J. I. & Polgar, L. (1965) J. Mol. Biol. 14, 630-633 Harris, J. I. & Waters, M. (1976) Enzymes 3rd Ed. 23, 1-50 Kirschner, K. (1971...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1979
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)37727-x